FastQCFastQC Report
Wed 25 May 2016
SRR1700220_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700220_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8035920
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA415330.5168418799589841No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA209140.26025644854602836No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT133430.1660419715477506No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT121830.15160678553295703No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC103420.12869714979740962No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA84700.10540174615974275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG118400.029.6864171
TACCTGG119900.029.0625512
ACCTGGG127300.026.7192883
TATAACG6200.023.9471472
ATAACGC7250.021.7200853
CCTGGGG91750.021.698954
GTACCCG11250.021.2976841
TACCCGG11300.020.9031812
TAACGCA7000.019.6034854
CTGGGGG48700.015.4283345
GTACCGG9150.015.3161521
GTACACG7900.015.1644921
TACACGG7400.014.895872
GTCTCGC9650.014.0540991
TCTGTCG11550.014.0241438
GTATAAC16900.013.9099551
TATACTG27450.013.5220675
ACACCGT7000.013.1761136
GTACATG166800.012.9686551
ACCCGGG17950.012.9084553
Produced by FastQC (version 0.11.5)