FastQCFastQC Report
Wed 25 May 2016
SRR1700219_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700219_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7811125
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81460.1042871545391989No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG131050.029.6798762
GTACCTG132750.029.5151351
ACCTGGG135300.028.1656463
ATAACGC7150.027.6873073
TAACGCA7650.025.5836144
GTACCCG14700.024.9160041
TATAACG7700.024.833182
TACCCGG14750.024.0973432
CCTGGGG91800.023.598684
GTATCAA148700.019.6593111
GGTATCA83650.019.2169481
GTACCGG9550.019.1761931
GTACACG7800.018.8408051
TACACGG8000.017.7155512
TACCGGG9250.016.7807522
CTGGGGG44950.016.415285
ATCAACG173450.016.1861823
TCAACGC174350.016.1284334
CAACGCA177050.015.93335
GTATAAC18600.015.9235211
Produced by FastQC (version 0.11.5)