FastQCFastQC Report
Wed 25 May 2016
SRR1700218_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700218_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7459347
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG112650.028.977551
TACCTGG112850.028.7064592
ACCTGGG115000.027.309033
CCTGGGG90150.022.0103744
TATAACG5250.019.282882
GTACACG7850.018.1424751
TACCCGG9900.017.9519082
ATAACGC5900.017.9210973
TAACGCA6100.017.3337524
GTATCAA165300.016.7665331
GTACCCG10850.016.668091
CTGGGGG50550.016.3331325
TACACGG8350.015.8958682
GGTATCA103600.015.3398531
GTATAAC13950.014.9086821
GTACCGG8200.014.8869441
GTACATG169100.014.8390131
TCAACGC190400.014.1315174
ATCAACG190850.014.0508573
TACACCG6800.013.8952055
Produced by FastQC (version 0.11.5)