FastQCFastQC Report
Wed 25 May 2016
SRR1700217_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700217_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7756804
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG124050.030.2328191
TACCTGG122450.030.057222
ACCTGGG130500.027.7376633
GTACCCG10400.023.4757481
CCTGGGG91150.023.0029534
GTACCGG10250.022.0549031
GGTATCA77650.021.4854241
TAACGCA5850.021.1510124
ATAACGC6950.020.0692543
TACCCGG11900.020.0393332
TATAACG6400.020.0363792
TACCGGG10350.019.7799592
GTATCAA137150.019.0377311
GTACACG7250.018.0850221
CTGGGGG45650.017.5441995
TACACGG7550.016.090562
ATCAACG162150.015.56683353
TCAACGC163650.015.5066314
CGACCGT4100.015.36379621
GTATTAG23450.015.3279231
Produced by FastQC (version 0.11.5)