FastQCFastQC Report
Wed 25 May 2016
SRR1700216_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700216_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13171041
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA192600.14622989936786318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTA13400.017.96460543
CGAACTA17150.016.13551929
GTACCTG68350.016.0085771
TAACGGC15300.015.73328736
GCGAACT17500.015.68359528
GTATCAA359200.015.6777341
CGGTACC15550.015.48075245
GTACCGG7050.015.3921051
TACCTGG68150.015.2510572
GTACATG377150.015.0394681
TCGCCCC19450.014.68964238
TACATGG362050.014.5153692
GGTATCA228250.014.3021821
TTAACGG16700.014.28010435
ACCGTCG1758.1916914E-714.142188
GCGGTAC17050.014.11880944
CGTAGCG4850.013.91564415
ACGGCCG17600.013.80505738
ACATGGG366700.013.7791583
TAGGACC20550.013.5748584
Produced by FastQC (version 0.11.5)