FastQCFastQC Report
Wed 25 May 2016
SRR1700215_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700215_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12259610
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130800.022.4021641
CGCGGTA16500.020.58866743
GTATCAA240500.019.0841871
CGGTACC19100.018.25717545
TAACGGC19400.017.8583736
GTACCTG67000.017.2777791
TTAACGG20550.017.18797935
AACGGCC20200.017.03973837
ACGGCCG20750.016.80491838
TCGCCCC26150.016.77589838
GCGGTAC21200.016.3425644
TACCTGG68050.016.3313352
GCGAACT18900.016.0692228
CGAACTA18950.016.02734429
TTTAACG22700.015.85736934
CCAACCG28300.015.81983443
ATCAACG283650.015.719683
TCAACGC285850.015.61454
GTACATG350400.015.4666631
CAACGCA290100.015.4012555
Produced by FastQC (version 0.11.5)