FastQCFastQC Report
Wed 25 May 2016
SRR1700214_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700214_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5318320
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG84000.032.198081
TACCTGG83600.031.6729532
ACCTGGG89700.028.7917353
GTACCGG5150.026.7855551
TATAACG4650.026.1251722
CCTGGGG57550.024.4321234
ATAACGC5450.023.9414163
GTACCCG6950.023.1021061
TAACGCA5500.022.9061374
GGTATCA53800.021.2689631
GTATCAA100150.020.706011
TACCGGG6000.019.8720652
TACCCGG7500.019.7970772
GTACACG5300.018.3472371
CTGGGGG30750.017.8513575
ATCAACG117750.017.0611743
TCAACGC118700.016.9818044
GTATAAC12200.016.8678461
CAACGCA120850.016.7169175
TACACGG5150.016.599492
Produced by FastQC (version 0.11.5)