FastQCFastQC Report
Wed 25 May 2016
SRR1700213_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700213_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9607110
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG118450.027.9814821
TACCTGG117550.027.6928312
ACCTGGG125700.025.2530143
TATAACG5650.020.3068792
CCTGGGG92250.019.7779164
GTACCCG12100.017.3767831
GTATCAA205150.017.2029611
ATAACGC7750.016.8363443
GTACCGG10100.016.5646461
TACCCGG11450.016.504232
TAACGCA7550.015.7926464
GTACACG7800.015.65204051
TACCGGG10600.014.6441682
GGTATCA128750.014.3992011
GTACATG237600.014.3206221
ATCAACG239500.014.211993
TCAACGC240850.014.16054
CTGGGGG50250.014.1474135
CGAACTA6850.014.1226929
CAACGCA244600.013.95265
Produced by FastQC (version 0.11.5)