FastQCFastQC Report
Wed 25 May 2016
SRR1700212_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700212_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7790171
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96470.12383553583098497No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA82920.1064418226506196No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG100050.029.144121
TACCTGG101250.028.3512692
ACCTGGG106900.026.1374683
CCTGGGG72500.021.4727544
TAACGCA4900.019.742044
GTACACG6200.018.6077381
GTATCAA192250.017.5321451
ATAACGC5550.017.429913
TATAACG5500.016.7701912
GGTATCA115450.016.4588871
TACACGG6750.016.3308642
GTACCCG9450.015.5595351
TACCCGG9500.015.1556042
CTGGGGG38550.014.9978285
TACCGGG7550.014.6004422
GTACCGG8150.014.4331411
ATCAACG228350.014.334473
TCAACGC230400.014.2557484
CAACGCA234450.014.0478695
AACGCAG240500.013.8722116
Produced by FastQC (version 0.11.5)