FastQCFastQC Report
Wed 25 May 2016
SRR1700211_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700211_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7217580
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA141200.19563343946308875No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98740.1368048570296415No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72810.10087868787044965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG110400.029.9893251
TACCTGG110700.029.6095772
ACCTGGG119250.026.883313
ATAACGC5500.022.9061243
CCTGGGG73250.022.481734
GTACCCG9550.021.0756661
TATAACG6000.020.996992
TAACGCA6400.020.0364674
GTACCGG7500.019.298051
TACCGGG6650.019.2829512
TACCCGG10100.019.155672
GTACACG7250.017.4680631
TCTGTCG12150.017.0357768
TACACGG7550.016.3883782
CGAACTA1803.925379E-916.24823429
CTGGGGG39150.015.28537755
GTATCAA233350.015.0701411
CTGTCGC14400.014.6864129
GTACATG181100.014.6353751
GTCTCGC12450.014.35001851
Produced by FastQC (version 0.11.5)