FastQCFastQC Report
Wed 25 May 2016
SRR1700210_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700210_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6638078
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA132490.19959090568083113No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72280.10888693986421973No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG99200.031.2371221
TACCTGG99950.030.7234542
ACCTGGG109050.027.6443143
TAACGCA4550.026.2054234
TATAACG5200.023.7946592
TACACGG6150.023.7770712
ATAACGC6100.023.6035233
GTACACG6500.023.3143651
GTACCCG8600.022.6184121
CCTGGGG72150.021.670814
GTACCGG6800.021.6205431
TACCCGG8950.020.3602052
CTGGGGG38750.016.7784865
GTATAAC11650.016.6968541
ACACGGG8450.016.5067543
CGACCGT3300.016.36202621
TACCGGG7600.015.6885372
CGCCGAC3700.015.20120618
GTACATG144350.013.9925471
GTATTAG18500.013.9378321
Produced by FastQC (version 0.11.5)