FastQCFastQC Report
Wed 25 May 2016
SRR1700209_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700209_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8147088
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG126800.031.0839581
TACCTGG126150.030.7091222
ACCTGGG135200.027.8880943
CCTGGGG95500.023.7689114
GTACCGG9450.021.7879811
TATAACG5700.020.9181142
GTATCAA138950.020.566111
TACCGGG9000.020.2471562
GGTATCA74200.019.912081
TACCCGG11900.019.2830032
GTACCCG12900.018.4164941
CTGGGGG49650.018.2149645
ATAACGC6800.017.8651393
GTACACG8650.017.002181
ATCAACG162400.016.7618063
TCAACGC164250.016.6141054
CAACGCA167600.016.282025
TATCAAC171900.016.0056042
TACACGG8500.015.8801212
AACGCAG175950.015.7394776