FastQCFastQC Report
Wed 25 May 2016
SRR1700208_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700208_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12461555
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA127550.023.8547271
GTATCAA242450.021.1245211
GTACCTG87450.017.2026161
ATCAACG294250.016.8965783
TCAACGC294700.016.8707794
TACCTGG88050.016.7608382
CAACGCA299850.016.6035275
TATCAAC310300.016.1675572
AACGCAG309600.016.1460426
GTACCGG8650.015.4300941
ACCTGGG94850.015.108633
GTACATG309250.014.3230531
TACATGG305750.013.8623132
ACGCAGA362150.013.7853557
CGCAGAG366650.013.5977558
GTATTAG32700.013.4210551
ACATGGG305900.013.2451613
GAGTACT224000.013.10714512
AGAGTAC372900.012.80261511
TATACTG44100.012.6513275
Produced by FastQC (version 0.11.5)