FastQCFastQC Report
Wed 25 May 2016
SRR1700207_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700207_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5708391
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA139090.24365885238064455No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71870.12590237774532262No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA66080.11575941451803144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG102100.030.2635121
TACCTGG102350.029.7828042
TATAACG6250.029.1554092
ACCTGGG105000.028.1101043
GTACCCG10050.026.7839871
ATAACGC6850.025.9450453
TAACGCA7050.025.5281184
CCTGGGG70550.024.29834
TACCCGG11050.024.0233672
GTACCGG7400.021.3973831
TACCGGG6950.020.7161622
GTACACG5300.020.0593261
CTGGGGG33850.018.6752155
GTATAAC15550.017.7469521
TACACGG6050.017.1047442
CGTATCG1459.694122E-817.0679210
GTCTCGC9000.016.5880681
TATCACG4750.016.1027282
CGACCGT1908.0272E-915.39339221
CTGGGGA37400.015.3386285
Produced by FastQC (version 0.11.5)