FastQCFastQC Report
Wed 25 May 2016
SRR1700206_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700206_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8413317
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA209010.24842758212961666No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107030.12721498548075627No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA100490.11944159479548912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG98300.026.390711
TACCTGG97600.026.2074832
ACCTGGG102400.024.232343
TATAACG5800.019.3935222
CCTGGGG78050.019.2252834
TAACGCA5800.018.2301254
ATAACGC6750.017.3308323
GTACCGG8000.016.1148431
GTACCCG9900.015.9920461
GTACACG8500.015.6990821
TACCCGG9750.015.4591232
TAGCGCG2152.593879E-914.6490484
GTACATG196550.014.5680651
TACACGG7600.014.50431252
CGCGTAA1108.5994997E-414.31747610
TACATGG196500.013.8413492
TATACTG28150.013.6658775
GTATCAA276750.013.5499631
TCGACGT1352.6561736E-413.33267612
ACATGGG194650.013.2333833
Produced by FastQC (version 0.11.5)