FastQCFastQC Report
Wed 25 May 2016
SRR1700205_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700205_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11406458
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA124410.10906979186702831No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG120000.026.9575441
TACCTGG119050.026.6257462
ACCTGGG118700.026.1168373
CCTGGGG79800.020.664424
ATAACGC7700.018.6986963
TACCCGG9400.018.4282072
TAACGCA7350.018.0587124
GTACCCG10600.017.9266411
TATAACG8100.017.7752292
GTACCGG8650.016.4759311
TACACGG8800.015.5943532
TCTATAC35950.014.6433043
TACCGGG7850.014.6157382
CTGGGGG45850.014.5236065
GTACATG179750.014.4099391
GTATCAA220950.014.2518341
ACACGGG9350.014.1958863
GTACACG10250.014.1247791
TATACCG4650.014.0303075
TACATGG181800.013.0798062
Produced by FastQC (version 0.11.5)