FastQCFastQC Report
Wed 25 May 2016
SRR1700204_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700204_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7566869
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90210.11921707644205284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG105350.029.7160871
TACCTGG105150.029.3325652
ACCTGGG112950.026.5500773
TATAACG5900.021.7342532
CCTGGGG78700.021.6964634
TAACGCA6050.020.0798424
ATAACGC5900.019.4464383
GTACCCG9550.019.1854381
TACCCGG9400.018.6676062
GTATCAA177750.016.8996831
CTGGGGG40750.016.120455
GGTATCA110450.016.0356351
GTACCGG9250.015.8950441
GTACACG6550.015.885711
TACCGGG8800.015.85005952
GTCGTAC2101.722583E-915.0799131
TAGACCG2001.5807018E-814.6229575
GTATTAG20050.014.3277971
GTATAAC15450.014.2014721
GTACATG157700.014.1858281
Produced by FastQC (version 0.11.5)