FastQCFastQC Report
Wed 25 May 2016
SRR1700203_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700203_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7297165
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA130770.1792065822822973No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG116550.031.9261441
TACCTGG117450.031.164142
ACCTGGG124200.029.0721443
GTACCCG9700.026.5843431
TACCCGG10400.024.2273162
CCTGGGG80350.023.9388794
GTACCGG7800.023.7800141
TATAACG5750.023.0836662
GTACACG6850.022.7851221
ATAACGC5700.022.1022643
TACACGG7150.020.7662722
TACCGGG8700.020.1695382
TAACGCA6250.020.1572654
CTGGGGG41000.018.9303575
TCTGTCG12900.016.3941368
GTCTCGC12700.016.029931
GTACATG131200.014.7236971
ACACGGG9950.014.6964993
GTATAAC14600.014.5635691
TACATGG129050.013.9983922
Produced by FastQC (version 0.11.5)