FastQCFastQC Report
Wed 25 May 2016
SRR1700202_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700202_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9955804
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA323420.32485573239489246No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA147720.1483757615155943No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT107920.10839908057651597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG92450.027.1081312
GTACCTG96200.026.643471
ACCTGGG92300.026.4697253
GTACCGG5250.021.5450081
CCTGGGG57600.020.4268074
GTACACG6100.020.3971181
TACCGGG5400.019.1639652
TACCCGG6800.018.52682
GTACCCG6850.018.1638571
TAACGCA6100.018.0712244
ATAACGC7000.017.6760793
TATAACG7150.016.675972
CTGTCGC11450.016.1123989
TCTGTCG10650.015.4214458
TACACGG7350.014.3857282
TGTCGCC12350.014.02734910
CTGGGGG30950.013.8106545
GTACATG154550.013.7153391
CGCGTAA1150.001204146313.693789536
ACCGGGG4600.013.6937213
Produced by FastQC (version 0.11.5)