FastQCFastQC Report
Wed 25 May 2016
SRR1700201_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700201_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4897343
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA182720.37310027090199727No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA87110.17787196036708067No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT60040.12259708989139623No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54920.11214244131971152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG90350.031.8465082
GTACCTG91900.031.755681
ACCTGGG93200.030.3174863
TAACGCA4850.026.903314
TATAACG4900.026.6287882
GTACCCG6850.026.090581
CCTGGGG62300.025.9272144
ATAACGC5650.025.8811973
TACCCGG7450.023.5536252
GTACCGG5900.023.006281
GTACACG5100.020.4049171
TACACGG5050.019.6011122
CTGGGGG31150.019.2829155
TACCGGG6250.019.077232
GTATAAC11400.017.860141
ACACGGG6250.017.637443
CGACCGT2053.6379788E-1217.55860521
CCGACGC2200.017.38490532
TACGACG800.00171358516.8725515
CTGGGGA33100.016.3797975
Produced by FastQC (version 0.11.5)