FastQCFastQC Report
Wed 25 May 2016
SRR1700200_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700200_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4329059
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA76360.17638937237861624No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57660.13319291790663976No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43810.10119982194744861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG68650.031.9292471
TACCTGG69850.031.3699512
ACCTGGG73100.029.113883
TATAACG3250.028.3806172
TAACGCA4000.026.434084
GTACCCG6150.025.3792881
CCTGGGG43600.024.0966534
ATAACGC4350.023.272853
TACCCGG6550.023.0120262
GTACCGG4650.022.8639011
TACCGGG4650.020.803522
CTGGGGG22550.016.7605825
GTCTCGC8200.015.9999861
GTATAAC9000.015.8344691
CGCACGA1451.8131122E-615.51595112
CGGTCGT2202.1827873E-1015.33963410
TACACGG4150.015.1785842
TATACTG15050.014.7987495
TCTGTCG8450.014.6439228
GTACGAC1705.907168E-714.5569413
Produced by FastQC (version 0.11.5)