FastQCFastQC Report
Wed 25 May 2016
SRR1700199_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700199_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5226061
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA97230.18604834501549064No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55160.10554794519237337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG67550.027.2886371
TACCTGG67050.027.0431272
ACCTGGG72100.024.6816563
TATAACG3350.019.4748382
GTACCCG5950.019.386651
GTACCGG5050.019.2586861
CCTGGGG47150.019.228994
TACCCGG6000.019.1222762
TAACGCA4200.017.1409074
GTACACG4200.016.1555421
ATAACGC5000.015.298263
TACCGGG5150.015.28908352
CGCGGTA2651.8189894E-1215.28098943
GTCTCGC6150.014.342971
GTACATG102050.014.3175631
TCTGTCG7900.014.2392958
CTGTCGC9700.013.9163439
TAACGGC2752.910383E-1113.90737636
TATACTG17850.013.8639695
CGACCGT2002.498E-713.49898121
Produced by FastQC (version 0.11.5)