FastQCFastQC Report
Wed 25 May 2016
SRR1700197_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700197_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8462948
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA350960.414701827306513No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA174100.20572027619689973No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT115800.13683175177255016No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT109450.12932845623061845No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC93090.10999713102337388No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90480.10691309931243817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC3500.014.7843698
GTACATG239600.014.0892431
TACATGG236350.013.75412
GTATAGG17000.013.7086271
TAGACCG2205.5262717E-813.2936955
ACATGGG239400.013.1749483
GTACCTG56900.013.122221
GAGTACT161700.012.96712712
TATAACG3500.012.8552892
TACCTGG55750.012.7918772
AGTACTT166750.012.45299213
AGAGTAC284950.012.34826511
CCTATAC14900.012.2299243
TAGACAG21950.012.1965645
TATACTG27000.012.0817355
CGGACCG2808.06358E-912.05183332
GTACACG5700.011.9083611
CGAACTA4350.011.89483729
ACCGTCG2654.8499714E-811.8857118
GTACTTT176100.011.74021514
Produced by FastQC (version 0.11.5)