FastQCFastQC Report
Wed 25 May 2016
SRR1700196_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700196_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5299886
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56110.10587020173641472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG98800.032.6519932
GTACCTG100750.032.465621
ACCTGGG103200.030.8020733
CCTGGGG75850.026.1595634
TATAACG4850.023.1924042
ATAACGC5350.022.706883
TAACGCA5300.022.4966324
GTACCCG8750.021.9741121
GTACACG5150.020.6438671
GTACCGG5700.020.636161
TACCCGG8850.020.3359382
TACACGG4900.020.201062
CTGGGGG39500.019.7059125
GGTATCA55350.019.289671
GTATCAA96450.019.0438271
TACCGGG5750.017.606062
ACCGTCG1301.1089731E-515.5753278
CTGGGGA39800.015.4876325
ATCAACG114200.015.483673
TCAACGC114750.015.38985354
Produced by FastQC (version 0.11.5)