FastQCFastQC Report
Wed 25 May 2016
SRR1700195_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700195_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6334791
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA96420.15220707360353325No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75640.11940409715174502No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG103850.031.0180071
TACCTGG103950.030.471662
ACCTGGG107200.028.8343353
GTACCCG8300.024.2561741
CCTGGGG71950.023.6691724
TAACGCA5650.023.4920834
ATAACGC6200.023.2223573
TATAACG6200.022.1338082
TACCCGG8600.021.7118912
GTACACG5400.020.5263941
TACACGG5850.019.9970282
GTACCGG6250.019.544481
TACCGGG5650.018.7140332
CTGGGGG37900.018.4603165
GTATAAC13450.015.6412341
GTATCAA166300.015.0035351
GTACATG136400.014.2790441
CCTGGGA44200.013.7423024
GTATTAG19450.013.4911421
TACATGG136600.013.4551752
Produced by FastQC (version 0.11.5)