FastQCFastQC Report
Wed 25 May 2016
SRR1700194_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700194_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12652353
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129910.10267655352328535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA159600.021.1304421
GTATCAA295850.019.27371
GCGAACT11800.018.11265628
CGCGGTA10850.018.03894643
CGAACTA11950.017.885329
CGGTACC11250.017.39756245
GTACCGG6450.016.832371
ATCAACG354250.015.6858753
GTACCTG68500.015.6843611
TAACGGC12350.015.66600636
TCGCCCC14150.015.58085538
TCAACGC357750.015.5701464
CAACGCA363150.015.3510095
TATCAAC372650.015.0622932
AACGCAG371950.015.0427886
GCGGTAC13350.014.99789944
TACCGGG6450.014.9978992
GTACATG381650.014.899231
TACCTGG67650.014.8316252
TTAACGG13450.014.55232535
Produced by FastQC (version 0.11.5)