FastQCFastQC Report
Wed 25 May 2016
SRR1700193_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700193_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5914848
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA107330.18145859369505352No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62660.10593678823192076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG94200.030.997971
TACCTGG94300.030.3699762
ACCTGGG97650.028.521753
CCTGGGG63100.022.568474
GTACACG6050.022.4312671
TAACGCA4950.022.269924
TACACGG6150.020.851032
TACCGGG5950.020.7956982
ATAACGC5650.020.3071753
GTACCCG7700.020.2682511
GTACCGG5950.019.7671621
TACCCGG7850.019.487992
TATAACG6000.017.997732
TCTGTCG10500.017.5696588
CTGGGGG34700.016.791835
ACACGGG7600.015.9848273
CTGTCGC12200.015.8590599
GTATAAC14150.015.6648971
GGACCGT4150.014.6368326
GTATCAA163100.014.3530531
Produced by FastQC (version 0.11.5)