FastQCFastQC Report
Wed 25 May 2016
SRR1700192_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700192_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7500675
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA87400.1165228462771684No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75510.10067093961543461No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG72900.025.7532061
TACCTGG72900.025.212462
ACCTGGG77300.022.9915523
GTACCGG5350.020.716821
GTACCCG4350.017.159531
TATAACG4800.016.8725972
TACCGGG5550.016.6192552
CCTGGGG47250.016.1881714
GTATCAA194600.015.45929051
TAACGCA5050.015.1463584
CGGTACC4250.014.8214245
ATAACGC5950.014.74583
GTACATG149900.014.6973191
CGCGGTA3700.014.59251743
CTGTCGC11750.014.5519929
GTCTCGC11050.014.124321
TACCCGG5450.014.03469852
TACATGG148550.013.9327182
ACATGGG145450.013.5491193
TCTGTCG11700.013.4604298
Produced by FastQC (version 0.11.5)