FastQCFastQC Report
Wed 25 May 2016
SRR1700191_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700191_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8078975
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA97970.12126538329429166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTA6400.016.87249643
CGTTCGG800.001713751816.87249624
GTATCAA216800.016.4777981
GTACATG233950.015.0764611
ATACCGT5100.014.9984256
TACATGG225500.014.7250872
GCCGTTA7450.014.49449315
GGTATCA136150.014.4902261
TAACGGC7300.014.484241536
CGTAGCG1251.3838282E-414.39786315
GTATTAG30500.014.3163981
CGAACTA7550.014.30348729
GCGAACT7600.014.20938528
TCGTTCG950.00538502514.20841723
CGGTACC7600.014.20841745
ACATGGG224850.014.0873893
CCAACCG9950.014.01802143
TACCGCG7550.014.0055848
TATAACG3101.8189894E-1213.7882772
ATCAACG254450.013.6598773
Produced by FastQC (version 0.11.5)