FastQCFastQC Report
Wed 25 May 2016
SRR1700190_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700190_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12677535
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA235660.18588787173531762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG311650.014.7228021
GTATCAA349800.014.3007431
TACATGG314400.014.1462712
TATACCG4450.013.6497065
GAGTACT191650.013.37088612
ACATGGG318350.013.3277373
ATACCGT11500.013.3030486
GTATTAG32800.013.2439351
AGAGTAC341400.012.95583111
TACACCG8550.012.8928685
TACCGTC8050.012.8560917
TAGCGCG8700.012.67127729
AGTACTT202800.012.53531313
TCAACGC400800.012.1857344
GTATAGA37350.012.1757331
ATCAACG400850.012.1729893
CAACGCA404650.012.0697945
GTACTTT209200.012.01183514
TATACTG42600.011.98769955
AACGCAG411650.011.8924386
Produced by FastQC (version 0.11.5)