FastQCFastQC Report
Wed 25 May 2016
SRR1700189_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700189_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5673152
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83610.14737838859244384No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67060.11820589330234761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG49750.025.7659551
TACCTGG49350.025.2553352
GGTATCA73750.023.7878911
ACCTGGG56550.021.6023793
GTATCAA142450.020.4096131
TATAACG3700.020.0651662
GTACCGG3650.019.8204421
TACCGGG3800.018.3530672
GTACACG5100.017.7315251
TAACGCA3850.016.9461734
ATCAACG172950.016.2861393
TCAACGC174450.016.1332074
GTACCCG3650.016.104111
TATCAAC179650.015.8288892
CAACGCA178500.015.7797625
CGCGGTA3000.015.74811543
AACGCAG184550.015.396566
CGACCGT2651.8189894E-1215.28172921
CCTGGGG32850.015.2037944
ATAACGC4900.015.1513813
Produced by FastQC (version 0.11.5)