FastQCFastQC Report
Wed 25 May 2016
SRR1700188_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700188_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4606493
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA64890.14086638143160102No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63330.13747985723629666No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48160.10454808028580527No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG60950.030.1238421
TACCTGG62100.029.2349912
ACCTGGG67300.026.474993
GTACACG3450.021.6283251
CCTGGGG50450.021.00324
TATAACG4300.019.8809052
TAACGCA4250.019.0563324
ATAACGC4550.018.7887523
TACCCGG4150.017.8890342
CTGGGGG26050.015.54499155
GTACCCG5250.015.0742861
GTATCAA126100.014.7933631
TACACGG5050.014.700892
GGTATCA84950.014.213661
GTACATG94800.013.6431841
GTATAAC10350.013.54501151
GTATTAG12800.013.2488841
TCTATAC9050.013.1750743
TACATGG93650.013.0921132
GTCTTAC7950.013.0833441
Produced by FastQC (version 0.11.5)