FastQCFastQC Report
Wed 25 May 2016
SRR1700187_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700187_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6448987
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89900.13940173859863572No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70600.10947455778713773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96300.021.372271
TTTCGCG550.003940902720.45237229
GTATCAA174100.020.1747531
GTACCTG41800.018.7212731
TACCTGG43350.017.5408442
ATCAACG211150.016.2375373
TCAACGC213100.016.1311824
TATCAAC216500.015.9088992
CAACGCA216750.015.8387795
AACGCAG222500.015.4800176
GTACCCG4150.014.7146931
TAACGCA3250.014.5365784
ACCTGGG50650.014.4798613
ACGCGCT1108.6023274E-414.3167717
ACTAGCG950.00538419514.20868640
CGCCGTA1656.9310536E-613.6344925
CCGGTCG3009.094947E-1213.498679
CGATCGG1000.00756129113.49856610
ACGCAGA256700.013.3917467
GAGTACT161500.013.38712212
Produced by FastQC (version 0.11.5)