FastQCFastQC Report
Wed 25 May 2016
SRR1700186_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700186_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6414282
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA136240.21240101386250246No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73440.11449449837097903No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA66680.10395551676711438No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG79300.026.0444261
TACCTGG78450.025.6368732
ACCTGGG81450.024.0851483
ATAACGC5350.019.7636873
TATAACG5100.018.967882
CCTGGGG63850.018.4274254
TACCGGG5400.017.9141082
GTACCGG6100.017.0586721
TAACGCA5500.015.9523934
GTATTAG20050.014.6671651
GTACACG6400.014.4917581
TAACGGC3150.014.28378736
GTACATG145850.014.1916161
TACCCGG6400.014.06049252
CTAGACG2306.702976E-913.6938044
GTATAAC12600.013.6445841
GTATCAA196400.013.5220911
GTATAGA19100.013.3832711
TACATGG146050.013.1237682
TACACCG4800.013.1232295
Produced by FastQC (version 0.11.5)