FastQCFastQC Report
Wed 25 May 2016
SRR1700185_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700185_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7525920
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91630.12175255649807598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106150.020.7517991
CGTAGCG1009.08065E-720.24720815
CGCGGTA5300.019.10164643
GTATCAA190900.018.765751
CGAACTA5400.017.08120329
AACGGCC6200.016.32871837
GCGAACT5950.016.2582628
TAACGGC6250.016.1980936
CGGTACC6300.016.06996245
GTACCTG34650.015.8606061
GGTACCG5700.015.7875076
CGGCCGC6950.015.53773639
ATCAACG226150.015.5187393
TCAACGC227400.015.4334334
CAACGCA232150.015.1176515
TATCAAC235000.015.0202692
CCAACCG7350.014.99832943
GCCGTTA6000.014.997931515
GTACCGG2900.014.8174121
AACGCAG237950.014.7775256
Produced by FastQC (version 0.11.5)