FastQCFastQC Report
Wed 25 May 2016
SRR1700184_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700184_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4565136
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57370.12566985956168666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG64400.029.6466141
TACCTGG63600.029.3589322
ACCTGGG69500.026.3486823
CCTGGGG48300.022.3104174
TATAACG3500.021.2111992
TAACGCA3650.020.3395064
ATAACGC3850.019.2829073
GTACACG4550.017.898211
GTATCAA110800.017.6193371
GGTATCA69300.017.30061
CTGGGGG24900.015.7206045
TATCACG3950.015.3775082
TCTGTCG7150.014.4745278
ATCAACG133800.014.2748273
TAGACCG950.00538444814.2084585
TCAACGC134300.014.2049324
TATCAAC136750.014.0820462
GTACATG106150.014.0437781
TACACGG5300.014.0073942
CAACGCA136600.013.9986945
Produced by FastQC (version 0.11.5)