FastQCFastQC Report
Wed 25 May 2016
SRR1700183_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700183_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6612805
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83840.12678432223542052No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA76730.11603245521378598No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68960.10428252458676765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119600.018.9006391
CCAACCG12800.016.69684443
GTATCAA196050.016.4537471
TCGCCCC12450.016.44344538
CGCCCCA13250.016.2995739
GCTCCGA14600.015.40981729
CGCGGTA7850.014.61575743
ATACCGT3750.014.399266
GTACCTG28250.013.9231811
GTACATG212050.013.6345221
GCCGTTA9600.013.59181715
CGGTACC8450.013.57795145
AGGTCGC15450.013.39676135
ATCAACG238100.013.322343
TACATGG205350.013.3107432
CGGATCG3051.2732926E-1113.27730337
GCTCGGA16350.013.2108311
TAGGACC11250.013.1981254
TCAACGC240350.013.1882644
CCGTTAA10000.013.04834216
Produced by FastQC (version 0.11.5)