FastQCFastQC Report
Wed 25 May 2016
SRR1700182_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700182_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5149895
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67190.13046867945851323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG72600.027.6704251
TACCTGG71200.027.6470582
ACCTGGG72650.026.4140033
TATAACG5050.025.3924332
ATAACGC5800.023.6604333
CCTGGGG48950.023.3930024
TAACGCA6000.021.7469124
GTACCCG4950.019.1947981
GGTATCA74800.018.358051
GTATCAA125450.018.0510651
TACCCGG5050.017.8192522
CTGGGGG26000.016.4399745
GTACCGG5350.015.6454261
TACCGGG4900.015.6100282
GTACACG4900.015.2355211
GTATAAC12550.015.1417131
ATCAACG146800.014.9876543
TCAACGC147500.014.947034
CAACGCA148750.014.8516725
TATCAAC152850.014.5416062
Produced by FastQC (version 0.11.5)