FastQCFastQC Report
Wed 25 May 2016
SRR1700181_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700181_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3304112
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA122220.3699027151621979No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA49050.14845138421457868No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45800.13861515590270548No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38710.11715704552388055No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT35770.10825904206637063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG46350.028.2918281
TACCTGG45750.028.078042
ATAACGC3250.026.9962233
TATAACG3300.025.9054662
ACCTGGG49350.025.3915823
TAACGCA4150.022.2258074
CCTGGGG35200.021.0268864
GTATAAC7050.018.6003721
GTACCGG3350.018.2222391
GTACACG3000.017.3336221
TACACGG3000.016.497692
ATACCGT1803.921741E-916.248716
TACCGGG3400.015.880132
GTACCCG3050.014.8256221
TACCCGG3200.014.7635592
CTGGGGG19800.014.4297995
ACACGGG3600.014.372993
CGGATCG1108.6024706E-414.31639637
TATCACG2752.910383E-1113.9071452
GATCGGA1301.9301378E-413.84421839
Produced by FastQC (version 0.11.5)