FastQCFastQC Report
Wed 25 May 2016
SRR1700180_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700180_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4475408
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70310.1571029948554411No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52120.11645865583651814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG58450.029.5673081
TACCTGG60800.028.3058872
ACCTGGG63850.026.143393
TAACGCA3900.025.3808984
TATAACG3750.024.5963972
GGTATCA51300.024.2961351
CGACCGT1353.6379788E-1223.32991621
ATAACGC4350.021.7209553
CCTGGGG42800.021.4980184
GTATCAA99100.021.3879361
GTACACG3950.020.0435161
GTACCCG4650.017.9991381
ATCAACG121600.016.965033
TACACGG4650.016.9330042
TCTGTCG10850.016.7955278
TCAACGC123150.016.7697724
GTCTCGC8250.016.7254891
ACACGGG4900.016.528193
CAACGCA125400.016.4868185
TATCAAC127800.016.3356362
Produced by FastQC (version 0.11.5)