FastQCFastQC Report
Wed 25 May 2016
SRR1700179_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700179_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2630338
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA41810.1589529558558634No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36160.13747282668615213No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29090.11059415177821254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG35300.026.4491351
ACCTGGG34200.025.7861083
TACCTGG36000.025.746642
TACACGG2000.023.6219162
CCTGGGG26550.019.7431854
GTACACG2800.017.7623631
TAACGCA2201.2732926E-1116.3615024
TAAACGC2101.07320375E-1016.07055333
CGCTTCG2251.8189894E-1115.99821537
GTCGCGC850.002567602815.8811858
GTAAACG2009.313226E-1015.74914332
GTATCAA78850.015.5393811
GTACCCG3400.015.292731
CGCACGA1351.5841912E-514.998898512
CTATGCG900.003757471514.9988979
TACGCGC900.003757471514.9988976
GTACCGG2900.014.811251
GTATTAG5800.014.811251
TACCCGG3200.014.7636982
TATAACG2751.8189894E-1214.7253492
Produced by FastQC (version 0.11.5)