FastQCFastQC Report
Wed 25 May 2016
SRR1700178_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700178_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4075833
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58390.1432590589457419No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45260.11104478520096382No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA43300.10623595225810283No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG51500.027.7827572
GTACCTG52050.027.714341
ACCTGGG54600.025.4224833
ATAACGC3450.024.1272643
TAACGCA3700.023.7130994
TATAACG4000.022.4970442
CCTGGGG39200.021.5214044
GTACACG3400.019.2851811
ACCGTCG851.2060437E-418.5281148
TACACGG3600.017.49772
GTATCAA108100.017.0883831
GTACCGG3900.016.2329731
GGTATCA68000.016.1929021
CTGGGGG21150.015.955355
GTATAAC8150.015.8132761
TACACCG2302.5465852E-1115.6501185
TAGCGCG1301.1086355E-515.5756429
ACACCGT2900.015.5155836
GTACCCG3200.014.8379511
GTACATG81650.014.4550281
Produced by FastQC (version 0.11.5)