FastQCFastQC Report
Wed 25 May 2016
SRR1700177_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700177_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10342071
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA215820.20868160738792066No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146430.1415867286155742No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA103820.10038608321292708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA327350.015.8408531
AGAGTAC335500.013.70603811
GAGTACT216650.013.551126512
GGTATCA215800.013.5477171
CGCGAAT1000.00755180913.50123628
GTACATG254350.013.3879971
ATAACGC6350.013.1089643
TCAACGC388950.013.0838334
ATCAACG389850.013.0826093
GTACCTG53400.013.0655841
CAACGCA394700.012.915595
TACATGG253650.012.9144322
TATCAAC399400.012.8770672
TATAACG5950.012.8561442
GTATTAG29250.012.8498771
TACCTGG52850.012.8135732
TAACGCA6150.012.8035114
AGTACTT226400.012.77868113
TATACTG39850.012.7585585
AACGCAG401950.012.7386626
Produced by FastQC (version 0.11.5)