FastQCFastQC Report
Wed 25 May 2016
SRR1700176_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700176_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11493891
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190000.1653052043037471No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143420.1247793284275969No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA142050.026.2855131
GTATCAA276500.021.3405651
ATCAACG355400.016.3889243
TCAACGC357600.016.2629014
CAACGCA361550.016.0636735
TATCAAC368100.015.8850842
AACGCAG369700.015.7096216
TAACGGC5250.014.56766136
GTATTAG34950.014.1284421
TTAACGG5400.013.74644535
ACGCAGA424550.013.6270847
CGCAGAG433350.013.3451698
GAGTACT257800.013.10532512
GTACATG330750.013.0887671
AGAGTAC419250.012.99993311
CGCGGTA5950.012.85365143
AGTACTT264800.012.60592813
TACATGG329600.012.5878932
ACCGTCG1801.7012795E-512.4964978
CGGTACC6550.012.43358645
Produced by FastQC (version 0.11.5)