FastQCFastQC Report
Wed 25 May 2016
SRR1700175_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700175_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6919377
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA162610.23500670652863692No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA80920.1169469447899717No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG38400.016.3072622
GTACCTG41200.015.5983041
GTATCAA224650.014.3234381
ACCTGGG43500.014.031993
GTACATG195650.013.7035071
CGCGGTA2002.505476E-713.49584443
TACATGG192800.013.4473222
AGAGTAC210950.013.275125511
GAGTACT110100.012.78897812
ACATGGG197000.012.6567043
GTATAGA22350.012.6113691
TATAACG3950.012.5456482
TATACTG25750.012.4912095
GTACCGT2756.184564E-912.2689496
CATGGGG121200.012.2096144
GGTATCA155750.012.1758031
CGGACGT1300.003030134112.11218136
ATCAACG262100.012.0481453
GTATAAA32050.012.030891
TCAACGC263150.012.0086314
Produced by FastQC (version 0.11.5)