FastQCFastQC Report
Wed 25 May 2016
SRR1700174_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700174_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8009489
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109080.13618846345877994No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81330.10154205842594952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84250.029.4139441
GTATCAA179200.023.0018981
TCAACGC228800.017.7779644
ATCAACG229350.017.7353293
CAACGCA232050.017.5061025
TATCAAC236750.017.248442
AACGCAG237600.017.1730256
ACGCAGA273450.014.86401757
CGCAGAG279200.014.5095618
ATACCGC5600.014.4600727
GAGTACT159350.013.76285412
AGAGTAC281000.013.76022511
GTACATG247700.013.654971
CAGAGTA296500.013.35192710
GCAGAGT302100.013.2533389
TACATGG245850.013.1102632
AGTACTT166300.013.0793913
TATACCG5850.013.07311256
GTACTTT171700.012.65494114
ACATGGG250150.012.5781343
Produced by FastQC (version 0.11.5)