FastQCFastQC Report
Wed 25 May 2016
SRR1700173_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700173_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11263546
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163390.1450608893504763No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117550.10436322628770726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110200.029.0949441
GTATCAA237350.023.207791
ATCAACG301350.017.8997573
TCAACGC303100.017.788984
CAACGCA308650.017.4759245
TATCAAC316600.017.1732582
AACGCAG318100.017.0629756
ACGCAGA369450.014.6548547
CGCAGAG374700.014.4555268
GTACATG291850.013.3655221
AGAGTAC386000.013.34471411
GAGTACT245150.013.31363412
GTACCTG56600.013.2257771
CGCGTTA1200.001661588213.12121540
GCAGAGT408950.013.1183519
CAGAGTA405700.013.0460210
TACATGG290950.012.7342652
TACCTGG57350.012.6458722
AGTACTT258050.012.56951713
GTATTAT38400.012.5068491
Produced by FastQC (version 0.11.5)