FastQCFastQC Report
Wed 25 May 2016
SRR1700172_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700172_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10989149
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95450.025.7467381
GTATCAA184450.021.3421041
ATCAACG227150.016.9557693
TCAACGC227250.016.9483094
CAACGCA230350.016.727155
AACGCAG239650.016.1813436
TATCAAC242150.016.0266532
GTACATG274550.014.5188581
GTACCTG57700.014.1059721
TACATGG273600.013.9950632
ACGCAGA280300.013.7865257
CGCAGAG284550.013.5568978
TACCTGG56350.013.5502662
TAACGCA6000.013.5140464
ACATGGG276100.013.4928363
ATAACGC7350.013.176963
GTCGTAC3450.013.0702091
AGAGTAC295000.012.43617211
TATAACG7450.012.39565852
GTATTAG30750.012.3911961
Produced by FastQC (version 0.11.5)