FastQCFastQC Report
Wed 25 May 2016
SRR1700171_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700171_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13130735
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189710.14447782245243695No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140700.107153179163238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA362150.019.551641
GGTATCA202650.019.4779511
ATCAACG445450.015.59211353
TCAACGC447850.015.5186154
TATCAAC456900.015.3299972
CAACGCA454650.015.27771955
AACGCAG461900.015.0915456
GTACATG382450.014.3053611
TACATGG377200.013.9716282
AGAGTAC473700.013.56172411
ACATGGG378000.013.453083
GAGTACT292600.013.43776912
ACGCAGA524900.013.2245057
CGCAGAG530800.013.03937158
AGTACTT301250.012.95480513
GTACTTT317800.012.15275814
TATACTG49650.012.0509385
GCAGAGT573450.011.9754369
CAGAGTA566350.011.93889510
CGAACTA6100.011.80134429
Produced by FastQC (version 0.11.5)