FastQCFastQC Report
Wed 25 May 2016
SRR1700170_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700170_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8900034
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106530.11969617194720829No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84550.026.803991
GTATCAA177600.023.1768441
ATCAACG223700.017.901273
TCAACGC224400.017.8850274
CAACGCA227550.017.6273575
AACGCAG235800.017.0966826
TATCAAC239200.016.9387872
ACGCAGA275400.014.6135827
CGCAGAG280100.014.3523078
CCGACGC2853.6379788E-1214.21319432
CGCGACT1301.9313331E-413.84369617
CTAGCGC1801.1259599E-613.7486439
AGAGTAC287850.013.32503811
GAGTACT183250.013.09877612
CAGAGTA303400.012.90161210
GCAGAGT310150.012.8601869
GTACATG218500.012.5349521
CGACGCC3457.2759576E-1212.39349133
AGTACTT191800.012.37397613
TACATGG215750.012.2522782
Produced by FastQC (version 0.11.5)